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ùe·HMW�ÇõV—å8ÞÊCŸ1c�µ Ž"©"S#R%i%> Ú .E¥³&£à\
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),^²æRUí‡©±âðOv¼s8OzC7C�ª»ìïøJæ:Q±¿‡«ü±_f!e!~"Ñ$G%# � õKtý�8v	øþ5\¢É*1zW–›W€ØìðIF •!Ø$  ñì7Ê|$-‘šý_k:m %c% ?–IW#6[ãÃ¨ ¹ €!>% 4 C X ˆ WÔÇ®ØÊ&â‰¾@Od”c">D÷ç È!¡%, & Ç SŒ½#+/&§;
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4é†Ùí—%·ä— ÷ Š"L#“$ù$¨%; | ao¶]AEê­	æ	*
4¡ï nH‚@]’®¬ÄÑr.FOm–ÿ	…+Êõˆm{ÂiŽ9RëòM k!¤"#Š#�#ò#/ F <"à†M
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÷tÞéÞ"¦ñÞO§Î:“Q’ìøÞ!o#1 �U³ï×_ª‰�áSvÚý	e+,ÎÁ©a'oƒð!%ÄV]mß  ¬ $!l"¹" #C#æ#$ì$%, ÉËéëBr¨ªJû(
é÷c.ZÇ×_%ø”öÿÕ×õ÷N~êàdŒˆ"æ"A#ì#a%r%+  Ëû–~Ä"	§	¿^æ‹¼),É&\jÙ<q�+wzü'6¡=„Ô ,!u!ù!8"C"( ì 4z$	o	á#LË¾�óò(0lþvºç@XÙòø@@)¼Ö”(!J!�!û!í" '�‡‘šN	´	r
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� ¥¯ÒuÃö4�e©i?â|¡Ø š"? ek~èôø©	ù	
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zýEõ¹DI’R¯ÒÕç_ Ââõasz£½Ñ3@å2:q$‹å‡£"5®£!q!^"#P#½#O$/ _ e • ž Ã sÖŸYcÍ®{²Öü	–-ÖG]ÿ½Ô·kq¡ªÏâ’§juîo!.!B!F!{!¦!]#–#  ± ð8å�Wé�Y˜ýº�ì‚Ò½¼†Ñ‘ "¾"x#ç$%)%J% ÚÒiÇ­ÙÐ·Ip¶ÝiŠ‰šÆ û w!Â!"–"7%2 7 t þhâ#zÇE	P
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T}î'
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q
Œ"K€ô¸CH/®æŸ¹ô?`y´ÐØd¨?ä9÷2>ŠÙ ©Õ× ì"\$"  ýáy€±ÆÕD	•�'èHoµÜ—€›¼ëÛ	!!""k#w#(%1%6%% I Ð ›S[Þ¡í9
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Plõ/Ì„ÄÌ)UÜ§_g©ú?<%+ … ëìL	U
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&óô·.?@ABG»å>xƒÙÚ8¦§¨øù�žŸÖ !""[$5%  :”  ›GACNNNNNNGTC,CCAGGCCTGG,GATCCGGATC,CCWGGTACCWGG,CCANNNNNTGG,CCAGGNNNTGG,CCANNNCCTGG,CCAGGNCCTGG,GCACNNNAACGTTNNNGTGC,CACNNNGTG,CCNNNNNNNGG,AACNAACRYGTGCGTGC,GCACGCACRYGTTNGTT,GCACNAACRYGTTNGTGC,AACGCACRYGTGCGTT,GCACNNNNNNGTTSAACNNNNNNGTGC,GACNNNNNGTC,GAANNNNNNNTTGG,CCAANNNNNNNTTC,CACNNNNGTG,AACACNNNNGTGC,GCACNNNNGTGTT,GCANNNNNNTGC,GAACNNNNNNTCC,GGANNNNNNGTTC,CAGNNNCTG,CCAGGNNCTG,CAGNNCCTGG,CCWGGGCCC,GGGCCCWGG,CCWGGGCCCWGG,AACNNNNNNGTGCACNNNNNNGTT,GACNNNNNNTTYG,CRAANNNNNNGTC,GACNNNGTC,GAANNNNTTC,CCWGGTACC,GGTACCWGG,CCWGGWCCWGG,ACNNNNGTAYC,GRTACNNNNGT,CCWGGYRCCWGG,GAAGNNNNNNTAC,GTANNNNNNCTTC,AACGGCNNNGTGC,GCACNNNGCCGTT,GCACGGCNNNGTT,AACNNNGCCGTGC,CGANNNNNNTGC,GCANNNNNNTCG,CGATCNNNNTGC,GCANNNNGATCG,TGANNNNNNTCA,TGATCNNNNTCA,TGANNNNGATCA,TGATCNNGATCA,AACCTGCNNGTGC,GCACNNGCAGGTT,GCACCTGCNNGTT,AACNNGCAGGTGC,GCCNNNNNGGC,AACNNNNNNGTGCMC,GKGCACNNNNNNGTT,CCWGGNCCWGG,AACTGGGNNGTGC,GCACNNCCCAGTT,GCACTGGGNNGTT,AACNNCCCAGTGC,GACNNNNGTC,GAGNNNNNCTC,CCWGGTCTC,GAGACCWGG,GATNNNNATC,GATCNNNATC,GATNNNGATC,GATCNNGATC,CCWGGCGCCWGG,ACNNNNNCTCC,GGAGNNNNNGT,AACNNNNNNGTGCAG,CTGCACNNNNNNGTT,GATCATGATC,CCWGGNNCCWGG,AACTGGNNNGTGC,GCACNNNCCAGTT,GCACTGGNNNGTT,AACNNNCCAGTGC,AACNAACCGGTGCGTGC,GCACGCACCGGTTNGTT,GCACNAACCGGTTNGTGC,AACGCACCGGTGCGTT,GCANNNNNTGC,GCACNNNNNNGTTCGAACNNNNNNGTGC,CCTNNNNNAGG,GCNNNNNNNGC,CCANNNNNNTGG,CCAGGNNCCTGG,CCTGGCCAGG,CAANNNNNGTGG,CCACNNNNNTTG,TTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAA,GCACNNNNNNGTTTAAACNNNNNNGTGC,CCAGGNCCY,RGGNCCTGG,CCWGGGNCCTGG,CCAGGNCCCWGG,AAGNNNNNCTT,GAYNNNNNVTC,GABNNNNNRTC,GAYNNNNNRTC,GATCNNNNVTC,GABNNNNGATC,GAYNNNNGATC,GATCNNNNRTC,GATCNNNGATC,GTYAACNNNNNNGTGC,GCACNNNNNNGTTRAC,GCACNNNNNNGTTAACNNNNNNGTGC,GATCNGATC,TAACTATAACGGTCCTAAGGTAGCGA,TCGCTACCTTAGGACCGTTATAGTTA,CTCTCTTAAGGTAGC,GCTACCTTAAGAGAG,TAGGGATAACAGGGTAAT,ATTACCCTGTTATCCCTA,GTTAACNNNNNNGTGC,GCACNNNNNNGTTAAC,AACNAACGCGTGCGTGC,GCACGCACGCGTTNGTT,GCACNAACGCGTTNGTGC,AACGCACGCGTGCGTT,TCCAACNNNNNNGTGC,GCACNNNNNNGTTGGA,CAYNNNNRTG,AACAYNNNNGTGC,GCACNNNNRTGTT,GTTTAAACNNNNNNGTGC,GCACNNNNNNGTTTAAAC,CCWGGCGCC,GGCGCCWGG,CCWGGNNCC,GGNNCCWGG,GATCGCGATC,AACNAACATGTGCGTGC,GCACGCACATGTTNGTT,GCACNAACATGTTNGTGC,AACGCACATGTGCGTT,TGGCAAACAGCTATTATGGGTATTATGGGT,ACCCATAATACCCATAATAGCTGTTTGCCA,ATCTATGTCGGGTGCGGAGAAAGAGGTAATGAAATGG,CCATTTCATTACCTCTTTCTCCGCACCCGACATAGAT,AACNNGCACGTGCNNGTT,GAACNNNNNCTC,GAGNNNNNGTTC,CCAGGWCCY,RGGWCCTGG,CCWGGGWCCTGG,CCAGGWCCCWGG,CCAGGWCCTGG,TTATAACNNNNNNGTGC,GCACNNNNNNGTTATAA,GCACNNNNNNGTTATAACNNNNNNGTGC,GAACNNNNNNTAC,GTANNNNNNGTTC,GGCCNNNNNGGCC,AACNNACTAGTGC,GCACTAGTNNGTT,AACTAGTNNGTGC,GCACNNACTAGTT,AACNAACTAGTGCGTGC,GCACGCACTAGTTNGTT,GCACNAACTAGTTNGTGC,AACGCACTAGTGCGTT,AACNNNAACGTGCNNGTGC,GCACNNGCACGTTNNNGTT,TTAACNNNNNNGTGC,GCACNNNNNNGTTAA,CACNNNNNNTCC,GGANNNNNNGTG,CACNNNNGATCC,GGATCNNNNGTG,GATCTAGATC,CCANNNNNNNNNTGG,CCAGGCCT,AGGCCTGG,CCTCGAGG,GATCCGGA,TCCGGATC,GGCGCGCC,GCGATCGC,CCWGGWCC,GGWCCWGG,GATCGATC,GATCATGA,TCATGATC,GCGGCCGC,CCTGGCCR,YGGCCAGG,CCWGGNCC,GGNCCWGG,GGCCGGCC,RTGCGCAY,GATCNNGA,TCNNGATC,CGCGCGCG,CCTGGCCA,TGGCCAGG,CGCCGGCG,GTTTAAAC,GATCGCGA,TCGCGATC,TTAATTAA,VCTCGAGB,CCTGCAGG,CRCCGGYG,CGTCGACG,ATTTAAAT,GCCCGGGC,GATCTAGA,TCTAGATC,CACCTGC,GCAGGTG,CCTNAGG,GATCGAT,ATCGATC,CCTCAGC,GCTGAGG,GCTNAGC,CCTNAGC,GCTNAGG,GCTCTTC,GAAGAGC,GGTNACC,CGGWCCG,ACCWGGT,RGGNCCY,CCTGGAG,CTCCAGG,GGTGATC,GATCACC,GATCNGA,TCNGATC,GGGWCCC,GAAGATC,GATCTTC,CCCWGGG,TCCNGGA,TCCWGGA,RGGWCCY,TTATAA,AGGCCT,GACGTC,GTMKAC,TCCGGA,TGCGCA,ACCTGC,GCAGGT,GGTACC,AGTACT,GGYRCC,CCGCTC,GAGCGG,AACGTT,YGGCCR,RAATTY,CTGAAG,CTTCAG,CACGTG,GRCGYC,AGCGCT,CTTAAG,ACRYGT,ACCGGT,ACTAGT,CACGTC,GACGTG,GWGCWC,GTGCAC,CYCGRG,GGGCCC,ATTAAT,CCTAGG,TTCGAA,GCTAGC,GKGCMC,TGGCCA,GGATCC,GRGCYC,ATCGAT,CACGAG,CTCGTG,GGCGCC,GAAGAC,GTCTTC,GCATGC,GTATCC,GGATAC,TGATCA,ACTGGG,CCCAGT,CTRYAG,RGCGCY,AGATCT,GGNNCC,CTGGAG,CTCCAG,CTTGAG,CTCAAG,CGATCG,GGTCTC,GAGACC,YACGTR,CCNNGG,GAATGC,GCATTC,WCCGGW,GCAATG,CATTGC,RCCGGY,GCGCGC,GAGGAG,CTCCTC,CGGCCG,CCCAGC,GCTGGG,GTGCAG,CTGCAC,CGRYCG,CGTACG,CGTCTC,GAGACG,CCATGG,TCGCGA,GDGCHC,TGTACA,TCATGA,ACATGT,GTATAC,CCWWGG,CTCTTC,GAAGAG,GCNNGC,CCRYGG,GTTAAC,RCATGY,TACGTA,RGATCY,GCGATG,CATCGC,GCAGTG,CACTGC,CCCGGG,CCGCGG,TTTAAA,GGCGGA,TCCGCC,GAGCTC,GATATC,CTGRAG,CTYCAG,CAGCAG,CTGCTG,GAATTC,ATGCAT,CATATG,GTYRAC,AAGCTT,GTNNAC,TCNNGA,CAATTG,ACGCGT,TCCRAC,GTYGGA,GCCGGC,CMGCKG,GCCGAG,CTCGGC,CTCGAG,CTGCAG,CAGCTG,GTCGAC,CTYRAG,AATATT,GATCGA,TCGATC,GACCGA,TCGGTC,WGTACW,TARCCA,TGGYTA,TCTAGA,GGATC,GATCC,TTSAA,CCWGG,GTCTC,GAGAC,GCWGC,GGNCC,CCSGG,GGTGA,TCACC,GGWCC,GCAGC,GCTGC,CCATC,GATGG,ACGGC,GCCGT,GCNGC,CCNGG,GCATC,GATGC,ACTGG,CCAGT,GGATG,CATCC,CTCAG,CTGAG,GGGAC,GTCCC,ACNGT,CTNAG,CAGTG,CACTG,GACGC,GCGTC,CCCGC,GCGGG,GANTC,CGWCG,TCNGA,CCTTC,GAAGG,GCTCC,GGAGC,GTNAC,GAAGA,TCTTC,GAGTC,GACTC,GTSAC,GAWTC,GCSGC,CASTG,ATGAA,TTCAT,ACGGA,TCCGT,CGCG,CCGC,GCGG,GTAC,AGCT,GCGC,CTAG,GATC,GGCC,CCGG,CATG,RGCY,YATR,ACGT,TGCA,CCDG,CHGG,AATT,CCTC,GAGG,TTAA,ASST,TCGA                                        .                                                                                                                                                                    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<SequenceClass>UNA</SequenceClass>
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<SequenceClass>UNA</SequenceClass>
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<SequenceClass>UNA</SequenceClass>
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<Created UTC="3:2:20">2023.6.7</Created>
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<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<SequenceClass>UNA</SequenceClass>
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<Created UTC="3:2:20">2023.6.7</Created>
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<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
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<Created UTC="3:2:20">2023.6.7</Created>
<LastModified UTC="3:2:58">2023.6.7</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
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<UUID>acce2039-680e-4b9b-afde-46607c8904e8</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<Created UTC="3:2:20">2023.6.7</Created>
<LastModified UTC="3:2:30">2023.6.7</LastModified>
<SequenceClass>UNA</SequenceClass>
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name="note"><V text="&lt;html&gt;&lt;body&gt;stem loop that binds the bacteriophage MS2 coat protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="23" name="MS2 stem loop" directionality="1" type="misc_RNA" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4196-4214" color="#99cc00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;stem loop that binds the bacteriophage MS2 coat protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="24" name="MS2 stem loop" directionality="1" type="misc_RNA" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4157-4175" color="#99cc00" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;stem loop that binds the bacteriophage MS2 coat protein&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="25" name="SV40 poly(A) signal" type="polyA_signal" swappedSegmentNumbering="1" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4349-4483" color="#a6acb3" type="standard"/><Q name="note"><V text="&lt;html&gt;&lt;body&gt;SV40 polyadenylation signal&lt;/body&gt;&lt;/html&gt;"/></Q></Feature><Feature recentID="26" name="ms2-polya" type="misc_feature" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1"><Segment range="4108-4483" color="#a6acb3" type="standard"/></Feature><Feature recentID="27" name="IL4" directionality="1" type="CDS" allowSegmentOverlaps="0" consecutiveTranslationNumbering="1" maxRunOn="4081" maxFusedRunOn="4081"><Segment range="3548-4006" color="#ffcc00" type="standard" translated="1"/><Q name="codon_start"><V int="1"/></Q><Q name="transl_table"><V int="1"/></Q><Q name="translation"><V text="MGLTSQLLPPLFFLLACAGNFVHGHKCDITLQEIIKTLNSLTEQKTLCTELTVTDIFAASKNTTEKETFCRAATVLRQFYSHHEKDTRCLGATAQQFHRHKQLIRFLKRLDRNLWGLAGLNSCPVKEANQSTLENFLERLKTIMREKYSKCSS"/></Q></Feature></Features>
   —<?xml version="1.0"?><Primers nextValidID="0"><HybridizationParams minContinuousMatchLen="10" allowMismatch="1" minMeltingTemperature="40"/></Primers>
  ³<Notes>
<UUID>e5cb23b1-940e-49cb-808a-57f452eff070</UUID>
<Type>Synthetic</Type>
<ConfirmedExperimentally>0</ConfirmedExperimentally>
<CustomMapLabel>pLV2-EF1a-IL4(human)-3Ã—FLAG-MS2-4X-Puro</CustomMapLabel>
<UseCustomMapLabel>1</UseCustomMapLabel>
<Created UTC="3:2:20">2023.6.7</Created>
<LastModified UTC="23:29:47">2025.9.20</LastModified>
<SequenceClass>UNA</SequenceClass>
<TransformedInto>unspecified</TransformedInto>
</Notes>
  Y     K         
?????                                                                                                       ÿ d      DO ÿþ               E              ÿÿÿÿYô? P                                                                                                                                                             k<StrandColors><TopStrand><ColorRange range="3507..3539" colors="black"/><ColorRange range="3540..3546" colors="green"/><ColorRange range="4006..4080" colors="green"/></TopStrand><BottomStrand><ColorRange range="3507..3539" colors="black"/><ColorRange range="3540..3546" colors="green"/><ColorRange range="4006..4080" colors="green"/></BottomStrand></StrandColors>   3<?xml version="1.0"?><EnzymeVisibilities vals=""/>
   )<?xml version="1.0"?><CustomEnzymeSets/>
